AFI #048 Joe E. Brown as Osgood Fielding III from Some Like It Hot (1959)

Osgood: I called Mama. She was so happy she cried! She wants you to have her wedding gown. It's white lace.
Daphne: Yeah, Osgood. I can't get married in your mother's dress. Ha ha. That-she and I, we are not built the same way.
Osgood: We can have it altered.
Daphne: Oh no you don't! Osgood, I'm gonna level with you. We can't get married at all.
Osgood: Why not?
Daphne: Well, in the first place, I'm not a natural blonde.
Osgood: Doesn't matter.
Daphne: I smoke! I smoke all the time!
Osgood: I don't care.
Daphne: Well, I have a terrible past. For three years now, I've been living with a saxophone player.
Osgood: I forgive you.
Daphne: [Tragically] I can never have children!
Osgood: We can adopt some.
Daphne/Jerry: But you don't understand, Osgood! [Whips off his wig, exasperated, and changes to a manly voice.] Uhhh, I'm a man!
Osgood: [Looks at him then turns back, unperturbed] Well, nobody's perfect!

- Joe E. Brown as Osgood Fielding III from Some Like It Hot (1959)

The bolded line is ranked #48 in the American Film Institute's list of the top 100 movie quotations in American cinema.