AFI #079 Ted Striker and Dr. Rumack from Airplane! (1980)

Striker: The stewardess told me that the pilot needed help...[Notices that only the autopilot is flying the plane] BOTH pilots?!
Rumack: Can you fly this plane and land it?
Striker: Surely you can't be serious.
Rumack: I am serious...and don't call me Shirley.

- Robert Hays as Ted Striker and Leslie Nielsen as Dr. Rumack from Airplane! (1980)

This next exchange is ranked #79 in the American Film Institute's list of the top 100 movie quotations in American cinema.